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Immunology and Infection

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JoVE Journal Immunology and Infection
Real-time Imaging of Leukotriene B4 Mediated Cell Migration and BLT1 Interactions with β-arrestin
Human T Lymphocyte Isolation, Culture and Analysis of Migration In Vitro
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Quantifying Myeloperoxidase-DNA and Neutrophil Elastase-DNA Complexes from Neutrophil Extracellular Traps by Using a Modified Sandwich ELISA
Journal (Immunology and Infection)
Quantifying Myeloperoxidase-DNA and Neutrophil Elastase-DNA Complexes from Neutrophil Extracellular Traps by Using a Modified Sandwich ELISA
 
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Real-time Imaging of Leukotriene B4 Mediated Cell Migration and BLT1 Interactions with β-arrestin

Article DOI: 10.3791/2315-v • 13:45 min • December 23rd, 2010
December 23rd, 2010 •
1, 1
1Microbiology and Immunology, James Graham Brown Cancer Center, University of Louisville

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This paper describes the methodology to determine the chemotactic response of leukocytes to specific ligands and identify interactions between the cell surface receptors and cytosolic proteins using live cell imaging techniques.

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Leukotriene B4 Cell Migration BLT1 β-arrestin G-protein Coupled Receptors Transmembrane Protein Family Extracellular Signals Intracellular Responses Chemotaxis Calcium Release Gene Regulation Ligand Binding Conformational Changes Heterotrimeric G-proteins Second Messengers Cyclic Adenosine Monophosphate (cAMP) Inositol Triphosphate (IP3) Diacyl Glycerol (DG) Desensitization Sequestration Internalization G-protein Receptor Kinases (GRKs) Cytosolic Proteins Membrane Translocation Pro-inflammatory Lipid Molecule Arachidonic Acid Pathway
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