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JoVE Journal
Biology

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从新生小鼠的肺血管内皮细胞的分离和培养
 
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从新生小鼠的肺血管内皮细胞的分离和培养

Article DOI: 10.3791/2316-v 10:47 min December 14th, 2010
December 14th, 2010

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

在这里,我们描述了一个小鼠肺血管内皮细胞的分离和培养协议。这种方法包括机械和酶的肺组织分解以及2步纯化过程中使用抗PECAM - 1和抗ICAM - 2的抗体结合磁珠,这将产生一个纯粹的原籍大多微血管的内皮细胞的人口。

Tags

细胞生物学,第46期,内皮细胞,肺,微血管细胞,鼠标,隔离,血管生成,血管通透性,粘附路口
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