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Visualizing Subcellular Localization of a Protein in the Heart Using Quantum Dots-Mediated Immuno-Labeling Followed by Transmission Electron Microscopy
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Biology
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Visualizing Subcellular Localization of a Protein in the Heart Using Quantum Dots-Mediated Immuno-Labeling Followed by Transmission Electron Microscopy

Visualizing Subcellular Localization of a Protein in the Heart Using Quantum Dots-Mediated Immuno-Labeling Followed by Transmission Electron Microscopy

DOI:
10.3791/64085-v

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08:13 min

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September 16, 2022

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, , , , , ,
1Department of Pathology and Translational Pathobiology,Louisiana State University Health Sciences Center-Shreveport, 2Department of Molecular and Cellular Physiology,Louisiana State University Health Sciences Center-Shreveport

Chapters

  • 00:05Introduction
  • 01:16Animal Preparation
  • 02:39Heart Tissue Processing and Embedding
  • 03:47Tissue Sectioning Using an Ultramicrotome and Ultrathin Heart Section Staining
  • 06:19Transmission Electron Microscopy Imaging
  • 06:48Results: Visualization of Sigmar1 Using Quantum Dots
  • 07:22Conclusion

Summary

Automatic Translation

Automatic Translation

The present protocol describes a method of immuno-labeling a protein in the heart tissue sections using quantum dots. This technique provides a useful tool to visualize any protein's subcellular localization and expression at the ultrastructural level.

Tags

Keywords: Subcellular LocalizationProteinHeartQuantum DotsImmunolabelingTransmission Electron MicroscopyFixationOsmicationContrast Enhancement

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