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JoVE Journal
Biology

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Counting Human Neural Stem Cells
 
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Counting Human Neural Stem Cells

Article DOI: 10.3791/262-v 06:38 min August 22nd, 2007
August 22nd, 2007

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

Knowledge of the exact number of viable cells is required for many tissue culture manipulations. This protocol describes how to differentiate between live and dead cells and quantify cells using a hemacytometer. Although it describes counting human neural stem/precursor cells (hNSPCs), it can be used for other cell types.

Tags

Counting Human Neural Stem Cells Viable Cells Cell Suspension Tissue Culture Manipulations Immunocytochemistry Cell Transfections Hemacytometer Live Cells Dead Cells Cell Concentration Total Cell Number Trypan Blue Quadrants Dilution Factor
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