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Visualisering av DNA Replication i Vertebrate Model System DT40 hjelp av DNA Fiber Technique
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Visualisering av DNA Replication i Vertebrate Model System DT40 hjelp av DNA Fiber Technique

Article DOI: 10.3791/3255-v • 07:18 min • October 27th, 2011
October 27th, 2011 •
1, 1,2
1Department of Molecular Oncology, Weatherall Institute of Molecular Medicine, University of Oxford, 2Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw

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DT40, en modell virveldyr genetisk system, gir et kraftig verktøy for å analysere protein funksjon. Her beskriver vi en enkel metode som gjør det mulig kvalitativ analyse av parametere som påvirker DNA syntese under S-fasen i DT40 celler på ett molekyl-nivå.

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Molecular Biology gener DNA fiber analyse replikasjon hastighet gaffel stalling opprinnelse avfyring oppsigelse
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