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Expression Analyse av pattedyrs Linker-histone Subtyper

Article DOI: 10.3791/3577-v • 14:40 min • March 19th, 2012
March 19th, 2012 •
1, 1, 1, 1
1School of Biology and the Parker H. Petit Institute of Bioengineering and Biosciences, Georgia Institute of Technology

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Vi beskriver et sett av metoder for å analysere uttrykk nivåer av H1 dennes histoner. mRNA av individuelle H1 gener kvantitativt måles ved tilfeldig primer basert revers transkripsjon etterfulgt av real-time PCR, mens protein kvantifisering av H1 histoner oppnås ved HPLC analyse.

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Genetikk H1 dennes histoner og histone H1 subtyper kromatin RT-PCR HPLC genekspresjon
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