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Immunology and Infection

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简单而强大在体内和在体外方法研究病毒大会

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Immunology and Infection

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JoVE Journal Immunology and Infection

Simple and Robust in vivo and in vitro Approach for Studying Virus Assembly

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简单而强大在体内和在体外方法研究病毒大会

DOI:

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09:47 min

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March 01, 2012

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Sonali Chaturvedi, Bongsu Jung, Sharad Gupta, Bahman Anvari, A.L.N. Rao

¹Department of Plant Pathology and Microbiology,University of California, Riverside , ²Department of Bioengineering,University of California, Riverside

Chapters

00:05Title
03:16Delivery and Expression of Functional Viral Genome Components to Plant Cells by Agroinfiltration
06:00Preparation of Capsid Protein Subunits for in vitro Assembly
07:07In vitro Assembly of RNA-containing Virions
08:48Representative Viral Assembly Results
09:22Conclusion

Summary

Automatic Translation

同步提供多个病毒成分对植物细胞通过一种简单，高效和可靠的方法<em>农杆菌</em>介导的瞬时表达描述。这种方法是适合就读复制，包壳其次<em>在体外</em>到非病毒部分基因组的重组耗尽适用于生物医学应用光学病毒鬼。

Tags

Virus Assembly In Vivo In Vitro Encapsidation Positive-sense RNA Genomes Infectious Virions Mechanism Regulating Virus Assembly Curbing Virus Spread Disease Control Macromolecular Interactions Subcellular Compartmentalization Biomedical Imaging Therapeutic Applications Non-enveloped Plant Viruses Brome Mosaic Virus (BMV)Genome Packaging Nicotiana Benthamiana Plants Agroinfiltration

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