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一种简单有效的方法构建突变牛痘病毒载体
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors
DOI:

09:16 min

October 30, 2016

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Chapters

  • 00:05Title
  • 00:49Preparing the CV-1 Cells
  • 02:32Purification of the Modified Vaccinia Virus (VV)
  • 05:23Expanding a Purified Plaque for PCR Testing
  • 06:52Results: VV N1L Modification
  • 08:28Conclusion

Summary

Automatic Translation

牛痘病毒(VV)已被广泛用于生物医学研究和人体健康的改善。本文介绍使用CRISPR-Cas9系统编辑VV基因组的简单,高效的方法。

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