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Chapters
- 00:05Title
- 01:29Human Fungiform Taste Papillae Digestion
- 02:59Culturing of Human Fungiform Taste Papillae Cells
- 05:36Freezing and Thawing Cultured Human Fungiform Taste Papillae Cells
- 07:09Results: Isolation and Culture of Human Fungiform Taste Papillae Cells
- 08:05Conclusion
Vi havde til formål at udvikle en reproducerbar protokol til at isolere og opretholde langsigtede kulturer af humane fungiform smag papiller celler. Celler fra menneskelige fungiform papiller opnået ved biopsi blev med succes holdt i kultur i mere end otte passager (12 måneder) uden tab af levedygtighed.
Tags
IsolationCultureHumanFungiform Taste Papillae CellsTaste CellsHistologicalMolecularPhysiological CharacteristicsDetectionSimple StimuliComplex Chemical MoleculesFoodsTaste BudsProtocolCulturingMultiple Cell CyclesMolecular PropertiesRegenerative PropertiesFunctional PropertiesSensory CellsPrimary CultureExplant CulturesRodentsSemi-intact Taste BudsLong-term CulturesViabilityTaste Cell ProliferationDifferentiation
