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JoVE Journal Biology
Kemisk blokeret antistof Microarray for Multiplexed Højkapacitetsforskning Profilering af specifikke protein Glycosylering i komplekse prøver
 
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Kemisk blokeret antistof Microarray for Multiplexed Højkapacitetsforskning Profilering af specifikke protein Glycosylering i komplekse prøver

Article doi: 10.3791/3791
May 4th, 2012 •
1, 1, 2, 1, 3, 4, 1
1Institute for Hepatitis and Virus Research, 2Department of Microbiology and Immunology, Thomas Jefferson University, 3Drexel University College of Medicine, 4Van Andel Research Institute

Summary
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May 4th, 2012

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I denne undersøgelse beskriver vi en forbedret protokol til et multiplekset high-throughput-antistof mikroarray med lectin påvisningsmetode, der kan anvendes i glycosylering profilering af specifikke proteiner. Denne protokol indeholder nye pålidelige reagenser og reducerer den tid, omkostninger og lab krav til udstyr i forhold til den tidligere procedure.

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