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JoVE Journal
Immunology and Infection

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Peptid-baserede identifikation af funktionskravene motiver og deres bindingspartnere
 
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Peptid-baserede identifikation af funktionskravene motiver og deres bindingspartnere

Article DOI: 10.3791/50362
June 30th, 2013

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Summary June 30th, 2013

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Teknikker til at dissekere mekanismerne bag udskillelsen af ​​HIV-1 Nef i exosomes beskrevet. Specifikke korte peptider afledt fra Nef og protein transfektion blev udnyttet til at bestemme strukturen, funktionen og bindingspartnere af Nef s Sekretion Ændring Region. Disse procedurer har generel relevans i mange mekanistiske undersøgelser.

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