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番茄/ GFP-FLP / FRT方法马赛克成人的实时成像<em>果蝇</em>感光细胞
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Biology
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JoVE Journal Biology
The Tomato/GFP-FLP/FRT Method for Live Imaging of Mosaic Adult Drosophila Photoreceptor Cells
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番茄/ GFP-FLP / FRT方法马赛克成人的实时成像<em>果蝇</em>感光细胞

DOI:
10.3791/50610-v

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09:33 min

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September 20, 2013

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, , , ,
1Laboratory of Molecular Biology of the Cell,Ecole Normale Supérieure de Lyon, 2INSERM U744, Institut Pasteur de Lille,Université Lille-Nord de France, 3Howard Hughes Medical Institute, Laboratory of Apoptosis and Cancer,The Rockefeller University

Chapters

  • 00:05Title
  • 02:14Preparing Drosophila for Photoreceptor Visualization
  • 04:29Visualizing the Photoreceptors
  • 06:01Time-course Visualization of Photoreceptor Cells
  • 07:14Results from a High-throughput Screen
  • 09:13Conclusion

Summary

Automatic Translation

Automatic Translation

番茄/ GFP-FLP / FRT方法涉及在活果蝇可视化马赛克感光细胞。它可以被用来追踪在视网膜上个别光感受器细胞命运数天或数周。这种方法是理想的视网膜变性和神经退行性疾病或光感受器细胞发育的研究。

Tags

DrosophilaPhotoreceptor CellsEyeClonal AnalysisLive ImagingTomato/GFP-FLP/FRTCornea NeutralizationMosaicNeuronal DegenerationPlanar Cell Polarity

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