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Implanting Glass Spinal Cord Windows in Adult Mice with Experimental Autoimmune Encephalomyelitis
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Implanting Glass Spinal Cord Windows in Adult Mice with Experimental Autoimmune Encephalomyelitis

Implanting Glass Spinal Cord Windows in Adult Mice with Experimental Autoimmune Encephalomyelitis

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7,301 Views

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09:20 min

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December 21, 2013

DOI:

10.3791/50826-v

DOI:
10.3791/50826-v

•

09:20 min
December 21, 2013

•

14 Views
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1Developmental Biology Institute of Marseille-Luminy (IBDML),Aix Marseille University, 2Campus de la Timone,European Research Center for Medical Imaging (CERIMED)

Summary

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We describe a method for implanting and maintaining glass windows over the exposed spinal cords of adult mice for studying experimental autoimmune encephalomyelitis. These windows provide chronic optical access to the spinal cord for monitoring cell dynamics at the subcellular level in live animals using two-photon microscopy.

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Cite this Article

Fenrich, K. K., Weber, P., Rougon, G., Debarbieux, F. Implanting Glass Spinal Cord Windows in Adult Mice with Experimental Autoimmune Encephalomyelitis. J. Vis. Exp. (82), e50826, doi:10.3791/50826 (2013).

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