Journal
/
Medicine
/
Implanting Glass Spinal Cord Windows in Adult Mice with Experimental Autoimmune Encephalomyelitis
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Implanting Glass Spinal Cord Windows in Adult Mice with Experimental Autoimmune Encephalomyelitis

Implanting Glass Spinal Cord Windows in Adult Mice with Experimental Autoimmune Encephalomyelitis

DOI:
10.3791/50826-v

•

09:20 min

•

December 21, 2013

•

, , ,
1Developmental Biology Institute of Marseille-Luminy (IBDML),Aix Marseille University, 2Campus de la Timone,European Research Center for Medical Imaging (CERIMED)

Chapters

  • 00:05Title
  • 01:05Performing the Laminectomy
  • 04:37Window Implantation
  • 06:30Postoperative Care and Imaging Tips
  • 06:59Neurodegeneration with EAE Induction
  • 08:21Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a method for implanting and maintaining glass windows over the exposed spinal cords of adult mice for studying experimental autoimmune encephalomyelitis. These windows provide chronic optical access to the spinal cord for monitoring cell dynamics at the subcellular level in live animals using two-photon microscopy.

Tags

Keywords: Intravital MicroscopySpinal CordExperimental Autoimmune Encephalomyelitis (EAE)Glass Window ImplantationThy1-CFP-23 MiceAxonal LossCell DynamicsAutoimmune Demyelinating Diseases

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/50826?article_type=v

Modeling Stroke in Mice – Middle Cerebral Artery Occlusion with the Filament Model
check_url/50826?article_type=v

Acute and Chronic Tactile Sensory Testing after Spinal Cord Injury in Rats
check_url/50826?article_type=v

Controlled Cervical Laceration Injury in Mice
check_url/50826?article_type=v

Evaluation of Respiratory Muscle Activation Using Respiratory Motor Control Assessment (RMCA) in Individuals with Chronic Spinal Cord Injury
check_url/50826?article_type=v

Controlled Cortical Impact Model for Traumatic Brain Injury
check_url/50826?article_type=v

A Radio-telemetric System to Monitor Cardiovascular Function in Rats with Spinal Cord Transection and Embryonic Neural Stem Cell Grafts
check_url/50826?article_type=v

Synergetic Use of Neural Precursor Cells and Self-assembling Peptides in Experimental Cervical Spinal Cord Injury
check_url/50826?article_type=v

Neural Stem Cell Transplantation in Experimental Contusive Model of Spinal Cord Injury
check_url/50826?article_type=v

Calibrated Forceps Model of Spinal Cord Compression Injury
check_url/50826?article_type=v

Contrast Enhanced Ultrasound Imaging for Assessment of Spinal Cord Blood Flow in Experimental Spinal Cord Injury

Read Article