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Journal / Immunology and Infection / Dissekere medfødte immunsignalisering i Viral…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Dissekere medfødte immunsignalisering i Viral Evasion av cytokinproduksion
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Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter
Journal (Immunology and Infection)
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Dissekere medfødte immunsignalisering i Viral Evasion av cytokinproduksion

Article DOI: 10.3791/51078-v • 08:32 min • March 2nd, 2014
March 2nd, 2014 •
1, 1, 1
1Department of Microbiology and Immunology, Keck School of Medicine, University of Southern California

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Vi beskriver en protokoll for å måle den antivirale cytokin produksjon i mus infisert med en modell herpesvirus, murine gamma herpesvirus 68 (γ HV68) som er nært beslektet med humant Kaposis sarkom assosiert herpesvirus (KSHV) og Epstein-Barr virus (EBV). Utnytte genmodifiserte mus stammer og mus embryonale fibroblaster (MEFs), vurderte vi de antivirale cytokin produksjon både in vivo og ex vivo. "Rekonstitusjon" uttrykk for medfødte immun komponenter i knockout embryonale fibroblaster ved lentiviral transduksjon, vi videre finne spesifikke medfødte immun molekyler og dissekere de viktigste signal hendelser som differentially regulerer antiviral cytokin produksjon.

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Immunologi , Cytokiner antivirale midler Medfødt gamma-HV68 mus infeksjon MEF antiviral cytokin
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