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Sanntidsovervåking av mitokondrie respirasjon i cytokin-differensierte humane primære T-celler
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Real-time Monitoring of Mitochondrial Respiration in Cytokine-differentiated Human Primary T Cells
DOI:

06:55 min

October 19, 2021

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Chapters

  • 00:05Introduction
  • 00:39Activating Human Primary T Lymphocytes
  • 01:55Extracellular Flux Assay
  • 04:52Results: Optimizing Mitochondrial Modulator Concentrations and Cell Numbers for Monitoring Mitochondrial Respiration in Human Primary T Cells
  • 06:26Conclusion

Summary

Automatic Translation

Metabolsk tilpasning er grunnleggende for T-celler da det dikterer differensiering, utholdenhet og cytotoksisitet. Her presenteres en optimalisert protokoll for overvåking av mitokondrie-respirasjon i ex vivo cytokin-differensierte primære T-celler.

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