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Immunology and Infection

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JoVE Journal Immunology and Infection
Dissekere multi protein signalnettverk komplekser av resultatet av dette Complementation affinitet rensing (BiCAP)
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Dissekere multi protein signalnettverk komplekser av resultatet av dette Complementation affinitet rensing (BiCAP)

Article DOI: 10.3791/57109-v • 06:45 min • June 15th, 2018
June 15th, 2018 •
1, 1, 1,2, 1,3, 1,4, 5, 1,6,7
1The Kinghorn Cancer Centre, Garvan Institute of Medical Research, 2Ubiquitin Signaling Group, Protein Signaling Program, The Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, 3RCSI Molecular Medicine, Royal College of Surgeons in Ireland, 4Department of Epigenetics, Max Planck Institute of Immunobiology and Epigenetics, 5School of Medical Sciences, University of New South Wales, 6St Vincent's Hospital Clinical School, University of New South Wales, 7School of Medicine and Medical Science, University College Dublin

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Dette manuskriptet beskriver protokollen for resultatet av dette Complementation affinitet rensing (BiCAP). Denne romanen metoden forenkler bestemt isolasjon og nedstrøms proteomic karakteristikk av noen to samspill proteiner, mens unntatt FN-kompleksbundet personlige proteiner som komplekser dannet med konkurrerende bindende partnere.

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Immunologi og infeksjon problemet 136 Protein-protein interaksjoner Proteomikk Interactomics BiCAP signaltransduksjon resultatet av dette fluorescens complementation Nanobody
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