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Bottom-up og Shotgun Proteomics at identificere en omfattende Cochlear Proteome
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Bottom-up og Shotgun Proteomics at identificere en omfattende Cochlear Proteome

Article DOI: 10.3791/51186-v • 14:24 min • March 7th, 2014
March 7th, 2014 •
1, 1
1Department of Otolaryngology, Morsani College of Medicine, University of South Florida

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Proteomanalyse af cochlear sensoriske epithel kan være en udfordring på grund af sin lille størrelse, og fordi membranproteiner er vanskeligt at isolere og identificere. Både membranen og opløselige proteiner, kan identificeres ved at kombinere flere præparative metoder og separationsteknikker sammen med høj opløsning massespektrometri.

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Biokemi Cochlear kromatografi LC-MS/MS massespektrometri Proteomics sensorisk epithel
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