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对于资源有限设置一个负担得起的HIV-1耐药性监测方法

JoVE Journal
Medicine
Author Produced

This content is Free Access.

JoVE Journal Medicine

An Affordable HIV-1 Drug Resistance Monitoring Method for Resource Limited Settings

Please note that all translations are automatically generated. Click here for the English version.

对于资源有限设置一个负担得起的HIV-1耐药性监测方法

DOI:

10.3791/51242-v

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19:57 min

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March 30, 2014

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Justen Manasa, Siva Danaviah, Sureshnee Pillay, Prevashinee Padayachee, Hloniphile Mthiyane, Charity Mkhize, Richard John Lessells, Christopher Seebregts, Tobias F. Rinke de Wit, Johannes Viljoen, David Katzenstein, Tulio De Oliveira

¹Africa Centre for Health and Population Studies, College of Health Sciences,University of KwaZulu-Natal, Durban, South Africa, ²Unit D11,Jembi Health Systems, ³Academic Medical Center, Department of Global Health, Amsterdam Institute for Global Health and Development (AIGHD),University of Amsterdam, ⁴Division of Infectious Diseases and Geographic Medicine, Centre for AIDS Research,Stanford Medical School

Chapters

00:00Title
00:48RNA Extraction
02:39Reverse Transcription
06:06PCR Purification
07:39Sequencing
11:11Bioinformatics
18:40Conclusion

Summary

Automatic Translation

耐药性检测为HIV-1感染的个体没有抗逆转录病毒疗法（ART）可以指导今后的治疗，提高治疗效果。在艾滋病高流行，但资源有限的环境优化个人和人群健康状况最终将需要负担得起的和可获得的耐药基因分型和解释方法。

Tags

HIV-1 Drug Resistance Antiretroviral Therapy Genotyping Resource-limited Settings Open-source Software Viral Load CRF02_AG Subtypes A And B

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