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Preparing Adult Drosophila melanogaster for Whole Brain Imaging during Behavior and Stimuli Responses
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Preparing Adult Drosophila melanogaster for Whole Brain Imaging during Behavior and Stimuli Responses

Preparing Adult Drosophila melanogaster for Whole Brain Imaging during Behavior and Stimuli Responses

DOI:
10.3791/61876-v

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07:51 min

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April 27, 2021

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, , ,
1Chair of Neuronal Control and Metabolism, School of Life Sciences,Technical University of Munich

Chapters

  • 00:05Introduction
  • 00:31Head Slot Creation
  • 01:44Fly Placement
  • 02:39Securing the Head
  • 03:36Body Positioning and Hole Sealing
  • 04:54Head Dissection
  • 05:59Results: Representative Drosophila melanogaster Whole Brain Imaging and Analysis
  • 07:14Conclusion

Summary

Automatic Translation

Automatic Translation

We present a method specifically tailored to image the whole brain of adult Drosophila during behavior and in response to stimuli. The head is positioned to allow optical access to the whole brain, while the fly can move its legs and the antennae, the tip of the proboscis, and the eyes can receive sensory stimuli.

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Keywords: Drosophila MelanogasterWhole Brain ImagingBehaviorStimuli ResponsesHead SlotBody Slot3D PrinterUV GlueProboscisTaste ExperimentsVisual Stimulation

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