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Assessing the Secretory Capacity of Pancreatic Acinar Cells
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JoVE Journal Biology
Assessing the Secretory Capacity of Pancreatic Acinar Cells

Assessing the Secretory Capacity of Pancreatic Acinar Cells

DOI:
10.3791/51799-v

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09:52 min

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August 28, 2014

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, ,
1Department of Molecular Genetics,Weizmann Institute of Science

Chapters

  • 00:05Title
  • 01:25Excision of the Murine Pancreas
  • 02:57Collagenase Digestion
  • 04:57Amylase Secretion Assay
  • 07:20Live Imaging of Pancreatic Secretion
  • 08:09Results: Assessing the Secretory Capacity of Pancreatic Acinar Cells
  • 09:10Conclusion

Summary

Automatic Translation

Automatic Translation

Isolated pancreatic acini retain their in vivo morphology and activity and offer powerful ways for monitoring and manipulating secretion. This work demonstrates how acini can be isolated from the mouse pancreas, and how their secretory capacities can be assessed.

Tags

Keywords: Pancreatic Acinar CellsSecretory CapacityDigestive EnzymesAcini IsolationAmylase Secretion AssayPancreatic Secretion Imaging

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