In this publication, we describe a rapid and convenient procedure for isolating and culturing primary pancreatic acinar cells from the murine pancreas. This method constitutes a valuable approach to study the physiology of fresh primary normal/untransformed exocrine pancreatic cells.
Gout, J., Pommier, R. M., Vincent, D. F., Kaniewski, B., Martel, S., Valcourt, U., Bartholin, L. Isolation and Culture of Mouse Primary Pancreatic Acinar Cells. J. Vis. Exp. (78), e50514, doi:10.3791/50514 (2013).