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Isolation and Culture of Mouse Primary Pancreatic Acinar Cells
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Isolation and Culture of Mouse Primary Pancreatic Acinar Cells

Isolation and Culture of Mouse Primary Pancreatic Acinar Cells

DOI:
10.3791/50514-v

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08:58 min

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August 13, 2013

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, , , , , ,
1INSERM U1052,Centre de Recherche en Cancérologie de Lyon, 2CNRS UMR5286,Centre de Recherche en Cancérologie de Lyon, 3Université de Lyon, 4Université Lyon 1, 5Centre Léon Bérard

Chapters

  • 00:05Title
  • 01:42Pancreas Dissection and Dilaceration
  • 03:51Enzymatic and Mechanical Dissociations of Pancreas
  • 05:46Filtration, Seeding of Dispersed Acini, and Acinar Cell Culture
  • 07:46Representative Acinar Culturing Results
  • 08:23Conclusion

Summary

Automatic Translation

Automatic Translation

In this publication, we describe a rapid and convenient procedure for isolating and culturing primary pancreatic acinar cells from the murine pancreas. This method constitutes a valuable approach to study the physiology of fresh primary normal/untransformed exocrine pancreatic cells.

Tags

Keywords: MousePancreatic Acinar CellsIsolationCultureExocrine PancreasIn VitroCell LinesTumorDifferentiation

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