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In vivo Mikroinjektion og Elektroporation af Mouse Testis

Article DOI: 10.3791/51802-v • 08:39 min • August 23rd, 2014
August 23rd, 2014 •
1, 1, 1
1Institute of Reproductive Biology, Leibniz Institute for Farm Animal Biology (FBN)

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Denne artikel beskriver mikroinjektion og elektroporation af muse testis in vivo som en transfektion teknik til testikulære museceller at studere unikke processer spermatogenesen. Den præsenterede protokol involverer trin glaskapillar forberedelse, mikroinjektion via efferente kanalen og transfektion ved elektroporation.

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Molekylærbiologi elektroporation transfektion mikroinjektion testikel sæd spermatogenesen reproduktion
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