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Chapters
- 00:05Title
- 01:04Sample Collection
- 01:46RNA Extraction Analysis
- 03:04Acrylamide Gel and Sample Preparation
- 03:58Electrophoretic Fractionation
- 04:41Gel Blotting
- 06:03Probe Synthesis, Membrane Crosslinking, and Prehybridization
- 07:27Membrane Hybridization, Washing, and Signal Detection
- 08:20Results: Improved RNA Detection Sensitivity
- 09:06Conclusion
Formålet med denne publikation er at visualisere og diskutere de operative trin i en Enhanced Northern blot protokol om RNA ekstraheret fra Drosophila melanogaster embryoner, celler og væv. Denne protokol er særligt anvendelige til effektiv detektering af små RNA-species.
