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增强Northern印迹检测小RNA种类的<em>果蝇</em
JoVE Journal
Biology
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JoVE Journal Biology
Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster
DOI:

09:39 min

August 21, 2014

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Chapters

  • 00:05Title
  • 01:04Sample Collection
  • 01:46RNA Extraction Analysis
  • 03:04Acrylamide Gel and Sample Preparation
  • 03:58Electrophoretic Fractionation
  • 04:41Gel Blotting
  • 06:03Probe Synthesis, Membrane Crosslinking, and Prehybridization
  • 07:27Membrane Hybridization, Washing, and Signal Detection
  • 08:20Results: Improved RNA Detection Sensitivity
  • 09:06Conclusion

Summary

Automatic Translation

本刊物的目的是可视化和探讨手术步骤,从果蝇的RNA提取的增强Northern杂交协议的果蝇胚胎,细胞和组织。这个协议是用于有效地检测小RNA种类是特别有用的。

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