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Biology
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增强Northern印迹检测小RNA种类的<em>果蝇</em
JoVE Journal
Biology
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JoVE Journal
Biology
Enhanced Northern Blot Detection of Small RNA Species in
Drosophila Melanogaster
Please note that all translations are automatically generated.
Click here for the English version.
增强Northern印迹检测小RNA种类的<em>果蝇</em
DOI:
10.3791/51814-v
•
09:39 min
•
August 21, 2014
•
Pietro Laneve
2
,
Angela Giangrande
1
Département de Génomique Fonctionnelle et Cancer
,
Institut de Génétique et de Biologie Moléculaire et Cellulaire
,
2
Center for Life NanoScience
,
Istituto Italiano di Tecnologia
Chapters
00:05
Title
01:04
Sample Collection
01:46
RNA Extraction Analysis
03:04
Acrylamide Gel and Sample Preparation
03:58
Electrophoretic Fractionation
04:41
Gel Blotting
06:03
Probe Synthesis, Membrane Crosslinking, and Prehybridization
07:27
Membrane Hybridization, Washing, and Signal Detection
08:20
Results: Improved RNA Detection Sensitivity
09:06
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本刊物的目的是可视化和探讨手术步骤,从
果蝇
的RNA提取的增强Northern杂交协议的
果蝇
胚胎,细胞和组织。这个协议是用于有效地检测小RNA种类是特别有用的。
Tags
Enhanced Northern Blot
Small RNA
MicroRNA
Drosophila Melanogaster
Gene Expression
Post-transcriptional Regulation
Noncoding RNA
RNA Profiling
RNA Detection
Molecular Biology
Embryo
Cell Culture
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