Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / Expressie van fluorescerende eiwitten in
JoVE Journal
Developmental Biology

A subscription to JoVE is required to view this content.

JoVE Journal Developmental Biology
Expressie van fluorescerende eiwitten in
A Standardized Approach for Multispecies Purification of Mammalian Male Germ Cells by Mechanical Tissue Dissociation and Flow Cytometry
Journal (Developmental Biology)
A Standardized Approach for Multispecies Purification of Mammalian Male Germ Cells by Mechanical Tissue Dissociation and Flow Cytometry
Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos
Journal (Developmental Biology)
Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos
 
Click here for the English version

Expressie van fluorescerende eiwitten in

Article DOI: 10.3791/52042-v • 09:31 min • January 12th, 2015
January 12th, 2015 •
1, 2, 1,3, 4, 1, 5, 2
1Département de Biologie du Développement et Cellules Souches, Institut Pasteur, 2Laboratoire de Biologie du Développement de Villefranche-sur-Mer (UMR7009 CNRS/UPMC Univ Paris 06), Sorbonne Universités, 3Equipe Epigenetic Control of Normal and Pathological Hematopoiesis, Centre de Recherche en Cancérologie de Marseille, 4Unité de Dynamique des Interactions Membranaires Normales et Pathologiques, CNRS UMR5235/DAA/cc107/Université Montpellier II, 5Plateforme BioEmergences IBiSA FBI, CNRS-NED, Institut de Neurobiologie Alfred Fessard

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Wij rapporteren hier de robuuste en efficiënte expressie van fluorescerende eiwitten na mRNA injectie in onbevruchte eicellen van Branchiostoma lanceolatum. De ontwikkeling van de micro-injectie techniek in deze basale chordate zal de weg voor vergaande technische innovaties in deze opkomende model systeem, met inbegrip van in vivo beeldvorming en gen-specifieke manipulaties effenen.

Tags

Developmental Biology Amphioxus Schedellozen genexpressie vectoren, micro-injectie protocol modelorganisme
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter