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Methods to Assess Subcellular Compartments of Muscle in C. elegans
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Methods to Assess Subcellular Compartments of Muscle in C. elegans

Methods to Assess Subcellular Compartments of Muscle in C. elegans

DOI:
10.3791/52043-v

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10:13 min

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November 13, 2014

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, , ,
1MRC/ARUK Centre for Musculoskeletal Ageing Research,University of Nottingham

Chapters

  • 00:05Title
  • 01:50Synchronization of L1 Larval Animals Using Gravity Flotation
  • 03:08Movement Assays
  • 03:59Imaging of Mitochondrial Networks and Sarcomeres in Body Wall Muscle and Assessing Membrane Potential
  • 06:13Assessment of Mucle Protein Degradation
  • 07:57Results: The Effect of Muscle Disprution in C. elegans
  • 09:37Conclusion

Summary

Automatic Translation

Automatic Translation

Skeletal muscle is essential for locomotion and is the bodies’ main protein store. Muscle health measurements within C. elegans are described. Prospective changes to muscle structure and function are assessed using localized GFP and cationic dyes.

Tags

Keywords: MuscleC. ElegansMitochondriaSarcomeresGFPCationic DyesMuscle FunctionMuscle StructureMuscle HomeostasisMovement AssaysGenomic RegulationIn Vivo

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