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方法来评估肌肉的亚细胞中<em>温度。线虫</em
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Methods to Assess Subcellular Compartments of Muscle in
C. elegans
Please note that all translations are automatically generated.
Click here for the English version.
方法来评估肌肉的亚细胞中<em>温度。线虫</em
DOI:
10.3791/52043-v
•
10:13 min
•
November 13, 2014
•
Christopher J. Gaffney
,
Joseph J. Bass
,
Thomas F. Barratt
,
Nathaniel J. Szewczyk
1
MRC/ARUK Centre for Musculoskeletal Ageing Research
,
University of Nottingham
Chapters
00:05
Title
01:50
Synchronization of L1 Larval Animals Using Gravity Flotation
03:08
Movement Assays
03:59
Imaging of Mitochondrial Networks and Sarcomeres in Body Wall Muscle and Assessing Membrane Potential
06:13
Assessment of Mucle Protein Degradation
07:57
Results: The Effect of Muscle Disprution in
C. elegans
09:37
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
骨骼肌的运动是必不可少的,是机构的主要蛋白质商店。
秀丽隐杆线虫
中的肌肉健康测量进行了描述。有意改变肌肉的结构和功能使用本地化的GFP和阳离子染料进行评估。
Tags
Muscle
C. Elegans
Mitochondria
Sarcomeres
GFP
Cationic Dyes
Muscle Function
Muscle Structure
Muscle Homeostasis
Movement Assays
Genomic Regulation
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