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JoVE Journal
Neuroscience

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Design, Surface Treatment, Cellular Plating, and Culturing of Modular Neuronal Networks Composed of Functionally Inter-connected Circuits
 

Design, Surface Treatment, Cellular Plating, and Culturing of Modular Neuronal Networks Composed of Functionally Inter-connected Circuits

Article DOI: 10.3791/52572-v 10:32 min April 15th, 2015
April 15th, 2015

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Summary

This manuscript describes a protocol to grow in vitro modular networks consisting of spatially confined, functionally inter-connected neuronal circuits. A polymeric mask is used to pattern a protein layer to promote cellular adhesion over the culturing substrate. Plated neurons grow on coated areas establishing spontaneous connections and exhibiting electrophysiological activity.

Tags

Keywords: Modular Neuronal Networks In Vitro Neuronal Circuits Network Patterning Surface Treatment Cellular Plating Culturing Functional Interconnectivity Micromachined Structures Biocompatible Polymers Electrophysiology Calcium Imaging
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