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JoVE Journal Neuroscience
Live-Imaging des Ependymale Cilia in der Seitenventrikel des Maus-Gehirn-
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Live-Imaging des Ependymale Cilia in der Seitenventrikel des Maus-Gehirn-

Article DOI: 10.3791/52853-v • 06:58 min • June 1st, 2015
June 1st, 2015 •
1, 1, 2, 3, 1
1Department of Pharmacology and Experimental Therapeutics, University of Toledo, College of Pharmacy and Pharmaceutical Sciences, 2Life Sciences Institute, University of Michigan, 3Department of Biomedical & Pharmaceutical Sciences, Chapman University, School of Pharmacy, Rinker Health Science campus

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Mit hochauflösenden Differentialinterferenzkontrast (DIC) Mikroskopie wird eine ex vivo Beobachtung des schlagenden motiler ependymaler Zilien innerhalb der Maus Hirnkammern entfernt von Live-Bildgebung nachgewiesen. Die Technik ermöglicht eine Aufnahme von der einzigartigen Zilienschlag Frequenz und Schlagwinkel sowie deren intrazellulären Calcium-Oszillation Stimulationseigenschaften.

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Neuroscience Ausgabe 100 frei bewegliche Zilien Seitenventrikel Liquor Live-Bildgebung Hydrocephalus.
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