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Immunology and Infection

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T-cellen Capture Bacteriën door Transinfection van dendritische cellen
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T-cellen Capture Bacteriën door Transinfection van dendritische cellen

Article DOI: 10.3791/52976-v • 11:39 min • January 13th, 2016
January 13th, 2016 •
1, 1,2, 1,2, 1,2, 1
1Cellular and Molecular Biology, Spanish National Research Council (CSIC), Spanish National Biotechnology Centre (CNB-CSIC), 2Hospital de Santa Cristina, Healthcare Research Institute Princesa Hospital (IIS-Princesa)

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Hier een protocol wordt bacteriële capture meten door CD4 + T-cellen die tijdens antigeenpresentatie via transinfection van pre-geïnfecteerde dendritische cellen (DC). We zien hoe de noodzakelijke stappen uit te voeren: het isoleren van primaire cellen, infectie van DC, DC / T-cel conjugaat vorming, en meting van bacteriële T celtransfectie.

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Immunologie transinfection antigeen presentatie T-cellen dendritische cellen bacteriën gentamicine survival test
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