Journal
/
/
使用拉姆齐试验来测量液体分泌和离子流率在<em>果蝇</em>马氏管
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Use of the Ramsay Assay to Measure Fluid Secretion and Ion Flux Rates in the Drosophila melanogaster Malpighian Tubule
DOI:

13:30 min

November 25, 2015

,

Chapters

  • 00:05Title
  • 00:53Preparing Tubule Transfer Rods
  • 01:33Preparing the Ion-Specific Electrodes
  • 03:38Preparing the Reference Electrodes and Calibrating the Electrodes
  • 07:16Tubule Dissection
  • 09:41Making Measurements
  • 11:06Results: Measurement K+ and Na+ Ion Fluxes to Investigate the Role of the Sodium-potassium-2-chloride Cotransporter (NKCC)
  • 12:07Conclusion

Summary

Automatic Translation

这个协议描述了使用的拉姆齐测定来测量流体分泌率从果蝇孤立马氏(肾)细管。此外,使用离子特异性电极来测量分泌的流体钠和钾的浓度,允许跨上皮离子通量的计算,进行说明。

Related Videos

Read Article