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Journal / Biology / Anställa Digital Droppe PCR för att upptäcka BRAF …
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Anställa Digital Droppe PCR för att upptäcka BRAF V600E Mutationer i formalinfixerade paraffininbäddade referensstandard cellinjer
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Anställa Digital Droppe PCR för att upptäcka BRAF V600E Mutationer i formalinfixerade paraffininbäddade referensstandard cellinjer

Article DOI: 10.3791/53190-v • 10:16 min • October 8th, 2015
October 8th, 2015 •
1, 3, 1, 3, 4, 2, 2, 1,2
1Research Institute of Pharmaceutical Science, Department of Pharmacy, College of Pharmacy, Seoul National University, 2The Center for Anti-Cancer CDx, N-Bio, Seoul National University, 3ABION CRO, 4ABION Inc., R&D Center

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Målet med den här videon är att visa hur man utför automatiserad DNA-extraktion från formalinfixerade paraffininbäddade (FFPE) referensstandard cellinjer och digital droppe PCR (ddPCR) analys för att upptäcka sällsynta mutationer i en klinisk miljö. Detektion av mutationer i FFPE proven visar den kliniska nyttan av ddPCR i FFPE proven.

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Molecular Biology DNA-mutation BRAF ddPCR molekylärbiologi Fluorescens FFPE cancerbiologi
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