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Lasergestuurde Neuronal Tracing In Brain Explantaten
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Lasergestuurde Neuronal Tracing In Brain Explantaten

Article DOI: 10.3791/53333-v • 06:40 min • November 25th, 2015
November 25th, 2015 •
1, 1
1Department of Physiology and Biophysics, University of Colorado School of Medicine

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We beschrijven een techniek voor neuronen en hun processen label via anterograde of retrograde tracer injecties in de hersenen kernen met een in vitro bereiding. We pasten een bestaande methode i n vitro tracer elektroporatie door gebruik te maken van fluorescent gelabelde muis mutanten en eenvoudige optische apparatuur om de nauwkeurigheid etikettering te verhogen.

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Neurowetenschappen kleuring en Labeling Neuroanatomische Tract-Tracing Technieken Elektroporatie Genotyping Technieken Neuro-anatomie neuroanatomie neuronale tracing tetramethylrhodamine choleratoxin subunit-b fluorescentie in-vitro elektroporatie optische genotypering
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