Modeling the Early Steps of Ovarian Cancer Dissemination in an Organotypic Culture of the Human Peritoneal Cavity

Pamela N. Peters; Elizabeth M. Schryver; Ernst Lengyel; Hilary Kenny

doi:10.3791/53541

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Modelleren van de eerste stappen van eierstokkanker Verspreiding in een Organotypische Cultuur van de menselijke buikholte

Modeling the Early Steps of Ovarian Cancer Dissemination in an Organotypic Culture of the Human Peritoneal Cavity

JoVE Journal
Medicine

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JoVE Journal Medicine

Modeling the Early Steps of Ovarian Cancer Dissemination in an Organotypic Culture of the Human Peritoneal Cavity

Modelleren van de eerste stappen van eierstokkanker Verspreiding in een Organotypische Cultuur van de menselijke buikholte

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26,540 Views

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09:00 min

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December 31, 2015

DOI:

10.3791/53541-v

DOI:

10.3791/53541-v

•

09:00 min

December 31, 2015

•

4 Views

Pamela N. Peters¹, Elizabeth M. Schryver¹, Ernst Lengyel¹, Hilary Kenny¹

¹Department of Obstetrics and Gynecology, Section of Gynecologic Oncology,University of Chicago

Summary

Here, we present a protocol to construct a three-dimensional in vitro model of the lining of the peritoneal cavity, composed of primary human mesothelial cells and fibroblasts layered with extracellular matrix, as a tool to investigate ovarian cancer cell adhesion, invasion, and proliferation.