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LC-MS Análise de plaquetas humanas como uma plataforma para estudar mitocondrial Metabolism
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LC-MS Análise de plaquetas humanas como uma plataforma para estudar mitocondrial Metabolism

Article DOI: 10.3791/53941-v • 06:04 min • April 4th, 2016
April 4th, 2016 •
*1,2, *2,3, 1,2, 2,3, 2,3, 4, 4, 1,2, 5, 1,2
1Center for Cancer Pharmacology, University of Pennsylvania, 2Center for Excellence in Environmental Toxicology, University of Pennsylvania, 3Penn SRP and Department of Systems Pharmacology and Translational Therapeutics, University of Pennsylvania, 4Division of Traumatology, Department of Surgery, Critical Care and Acute Care Surgery, University of Pennsylvania, 5A.J. Drexel Autism Institute, Drexel University
* These authors contributed equally

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Aqui mostramos plaquetas humanas isoladas podem ser usadas como uma acessível ex vivo modelo para estudar adaptações metabólicas em resposta ao inibidor do complexo I rotenona. Esta abordagem emprega rastreio isotópica e quantificação relativa por espectrometria de massa de cromatografia de líquido e pode ser aplicado a uma variedade de modelos de estudo.

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Ciências do Ambiente edição 110 Metabolismo traçadores isotópicos plaquetas espectrometria de massa as mitocôndrias rotenona xenobióticos
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