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Journal / Immunology and Infection / Et-trins Negativ kromatografisk oprensning af
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Immunology and Infection

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JoVE Journal Immunology and Infection
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Et-trins Negativ kromatografisk oprensning af

Article DOI: 10.3791/54043-v • 10:44 min • June 18th, 2016
June 18th, 2016 •
1, 1, 1, 1, 1,2
1Institute of Molecular and Cellular Biology, National Tsing Hua University, 2Department of Life Science, National Tsing Hua University

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Et højt udbytte fremgangsmåde til ettrins negativ oprensning af rekombinant Helicobacter pylori neutrofil-aktiverende protein (HP-NAP) overudtrykkes i Escherichia coli ved hjælp af diethylaminoethyl harpikser i batchvis, er beskrevet. HP-NAP renset ved denne metode er til gavn for udviklingen af vacciner, lægemidler, eller diagnostik for H. pylori-associeret sygdomme.

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Immunologi HP-NAP, DEAE Negativ kromatografi ubundne fraktion Batch kromatografi,
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