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Dissekere multi protein Signaling komplekser af Bimolecular komplementering affinitet rensning (BiCAP)
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Dissecting Multi-protein Signaling Complexes by Bimolecular Complementation Affinity Purification (BiCAP)
DOI:

06:45 min

June 15, 2018

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Chapters

  • 00:04Title
  • 00:45Plasmid Cloning
  • 02:54Cell Culture and Transfection
  • 03:41Harvesting Lysates
  • 04:31Affinity Purification and Preparation of BiCAP Eluant for Western Blotting
  • 05:22Results: BiCAP Method is a Powerful Technique to Specifically Purify and Characterize Interacting Proteins
  • 06:06Conclusion

Summary

Automatic Translation

Dette manuskript beskriver protokollen for Bimolecular komplementering affinitet rensning (BiCAP). Denne nye metode letter specifikke isolering og downstream proteom karakterisering af enhver to interagerende proteiner, mens bortset fra FN-kompleksbundet individuelle proteiner samt komplekser dannet med konkurrerende bindende partnere.

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