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JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology

בידוד של Murine העוברי Hemogenic לתאי אנדותל

Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells

Journal (Developmental Biology)

Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells

2D and 3D Human Induced Pluripotent Stem Cell-Based Models to Dissect Primary Cilium Involvement during Neocortical Development

Journal (Developmental Biology)

2D and 3D Human Induced Pluripotent Stem Cell-Based Models to Dissect Primary Cilium Involvement during Neocortical Development

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בידוד של Murine העוברי Hemogenic לתאי אנדותל

Article DOI: 10.3791/54150-v • 08:56 min • June 17th, 2016

June 17th, 2016 •

Jennifer S. Fang*¹, Emily C. Gritz*², Kathrina L. Marcelo³, Karen K. Hirschi¹

¹Departments of Medicine, Genetics and Biomedical Engineering, Yale Cardiovascular Research Center, Vascular Biology and Therapeutics Program, Yale Stem Cell Center, Yale University School of Medicine, ²Department of Pediatrics, Section of Neonatal-Perinatal Medicine, Yale University School of Medicine, ³Department of Molecular and Cellular Biology, Baylor College of Medicine

* These authors contributed equally

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גזע אבות היווצרות הדם תאים (HSPC) נובעים מתאי אנדותל מיוחדים (hemogenic) במהלך הפיתוח, עדיין מעט מאוד ידוע על התהליך שבו כמה תאי אנדותל לציין להפוך להרכיב דם. אנו מדגימים שיטה המבוססת זרימת cytometry המאפשרת בידוד סימולטני של תאי hemogenic האנדותל HSPC מרקמות עובריות בעכברים.

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ביולוגיה התפתחותית גיליון 112 האנדותל hemogenic hematopoiesis הסופי פיתוח כלי דם עוברי עכברים FACS תאי גזע אבות היווצרות דם

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