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JoVE Journal Developmental Biology
Kinetic Måling og Real Time Visualisering af Somatisk Omprogrammering
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Kinetic Måling og Real Time Visualisering af Somatisk Omprogrammering

Article DOI: 10.3791/54190-v • 08:56 min • July 30th, 2016
July 30th, 2016 •
1, 1, 1, 1
1Life Sciences Solutions, Thermo Fisher Scientific

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Protokollen præsenteres i denne undersøgelse beskriver fremgangsmåder til tidstro overvågning af omprogrammering progression via den kinetiske måling af positive og negative pluripotente stamceller cellemarkører ved anvendelse af flowcytometri analyse. Protokollen omfatter også imaging-vurdering af morfologi, og markør eller reporter udtryk under iPSC generation.

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Developmental Biology OMPROGRAMMERING inducerede pluripotente stamceller tidstro billeddannelse flowcytometri pluripotente stamceller cellemarkører kinetisk måling
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