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Biochemistry

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JoVE Journal Biochemistry
In linea Size-esclusione e cromatografia a scambio ionico su una SAXS Beamline
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In linea Size-esclusione e cromatografia a scambio ionico su una SAXS Beamline

Article DOI: 10.3791/54861-v • 11:09 min • January 5th, 2017
January 5th, 2017 •
1, 2,3, 4
1Structural Biology Group, European Synchrotron Radiation Facility, 2European Molecular Biology Laboratory, 3School of Chemical and Physical Sciences, Keele University, 4Groupe de Microscopie Electronique et Méthodes, Institut de Biologie Structurale

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La determinazione della struttura in soluzione di una proteina da piccolo angolo X-ray Scattering (SAXS) richiede campioni monodisperse. Qui, vi presentiamo due possibilità per garantire ritardi minimi tra la preparazione del campione e l'acquisizione dei dati: in linea cromatografia dimensione esclusione (SEC) e in linea cromatografia a scambio ionico (IEC).

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Biochimica Bio-Small Angle X-ray Scattering (BioSAXS) in linea cromatografia di esclusione molecolare (SEC) in linea cromatografia a scambio ionico (IEC) sottrazione del background D5 vaccinia
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