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Journal / Developmental Biology / Rumlig og tidsmæssig analyse af Active ERK i
JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology
Rumlig og tidsmæssig analyse af Active ERK i
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Rumlig og tidsmæssig analyse af Active ERK i

Article DOI: 10.3791/54901-v • 08:40 min • November 29th, 2016
November 29th, 2016 •
1, 1,2
1Program in Developmental Biology, Baylor College of Medicine, 2Department of Genetics, UT MD Anderson Cancer Center

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Vi præsenterer en immunfluorescens imaging-metode til rumlig og tidsmæssig lokalisering af aktiv ERK i dissekeret C. elegans gonade. Den her beskrevne protokol kan tilpasses til visualisering af enhver signal- eller strukturelle protein i C. elegans gonade, forudsat en egnet antistofreagens er tilgængelig.

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Developmental Biology RAS-ERK, Kimcellelinje, immunofluorescens signalproteiner
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