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Journal / Immunology and Infection / Genomweite Analyse von…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Genomweite Analyse von HDAC-Inhibitor-vermittelten Modulation der Micro-RNAs und mRNAs in B Zellen induziert unterziehen Class-Schalter DNA-Rekombination und Plasma-Zell-Differenzierung
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Genomweite Analyse von HDAC-Inhibitor-vermittelten Modulation der Micro-RNAs und mRNAs in B Zellen induziert unterziehen Class-Schalter DNA-Rekombination und Plasma-Zell-Differenzierung

Article DOI: 10.3791/55135-v • 11:06 min • September 20th, 2017
September 20th, 2017 •
1, 1,2, 1,3, 1,3, 1, 1
1Department of Microbiology, Immunology and Molecular Genetics, University of Texas Long School of Medicine at San Antonio, 2Xiangya School of Medicine, Cental South University, 3Greehey Children’s Cancer Research Institute, University of Texas Long School of Medicine at San Antonio

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Epigenetische Faktoren können mit genetischen Programme zu modulieren Genexpression und regulieren die Funktion der B-Zellen interagieren. Durch die Kombination von in-vitro- B-Zell-Stimulation, qRT-PCR, und Hochdurchsatz-RNA-Sequenz und mRNA-Sequenz Ansätze, analysieren wir die epigenetische Modulation von MiRNA und gen-Expression in B-Zellen.

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Immunologie Ausgabe 127 B-Zellen-Klasse-Schalter DNA-Rekombination Durchflusszytometrie Histon-Deacetylase (HDAC)-Inhibitoren (HDIs) mRNA MicroRNA mRNA-Seq MiRNA-Seq Plasma-Zell-Differenzierung
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