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在斑马鱼胚胎人乳腺癌细胞的侵袭行为
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
Invasive Behavior of Human Breast Cancer Cells in Embryonic Zebrafish
Please note that all translations are automatically generated.
Click here for the English version.
在斑马鱼胚胎人乳腺癌细胞的侵袭行为
DOI:
10.3791/55459-v
•
09:22 min
•
April 25, 2017
•
Jiang Ren*
1
,
Sijia Liu*
1
,
Chao Cui
,
Peter ten Dijke
1
Department of Molecular Cell Biology, Cancer Genomics Centre Netherlands
,
Leiden University Medical Center
Chapters
00:05
Title
01:28
Preparation of Fluorescent Cells
02:42
Preparation of Zebrafish Embryos
03:20
Xenotransplantation into the Perivitelline Space
05:07
Xenotransplantation into the Duct of Cuvier
05:47
Invasion and Metastasis Assessment
06:32
Results: Xenotransplanted MDA-MB-231, MCF-10A, and MCF-10A-Ras Cells Reveal Distinct Invasiveness Patterns
08:17
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们将介绍使用两个不同的注射部位,
即
卵周隙和居维叶管道,调查侵入行为,并分别评估人类乳腺癌细胞的血管内和外渗潜力,异种移植模型斑马鱼。
Tags
Invasive Behavior
Human Breast Cancer Cells
Embryonic Zebrafish
Metastasis
Intravasation
Extravasation
Anti-metastatic Activity
Peritumoral Angiogenesis
Cancer Cell Dormancy
MDA-MB-231 Cells
MCF10A Cells
MCF10A-Ras Cells
Cell Implantation
Microinjection
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