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在斑马鱼胚胎人乳腺癌细胞的侵袭行为
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Invasive Behavior of Human Breast Cancer Cells in Embryonic Zebrafish
DOI:

09:22 min

April 25, 2017

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Chapters

  • 00:05Title
  • 01:28Preparation of Fluorescent Cells
  • 02:42Preparation of Zebrafish Embryos
  • 03:20Xenotransplantation into the Perivitelline Space
  • 05:07Xenotransplantation into the Duct of Cuvier
  • 05:47Invasion and Metastasis Assessment
  • 06:32Results: Xenotransplanted MDA-MB-231, MCF-10A, and MCF-10A-Ras Cells Reveal Distinct Invasiveness Patterns
  • 08:17Conclusion

Summary

Automatic Translation

在这里,我们将介绍使用两个不同的注射部位, 卵周隙和居维叶管道,调查侵入行为,并分别评估人类乳腺癌细胞的血管内和外渗潜力,异种移植模型斑马鱼。

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