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JoVE Journal
Biochemistry

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对酶活性G4 Resolvase1净化用G-四DNA亲和方法
 

对酶活性G4 Resolvase1净化用G-四DNA亲和方法

Article doi: 10.3791/55496
March 18th, 2017

Summary March 18th, 2017

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G4 Resolvase1结合G-四(G4)的结构与一个G4结合蛋白的紧密报道的亲和力,并表示在HeLa细胞中的大多数G4-DNA解旋活动。我们描述了线束的亲和力和G4-Resolvase1的ATP依赖的平仓活动,特别是净化催化活性的重组G4R1一个新的协议。

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