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JoVE Journal
Biochemistry

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A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1
 

A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1

Article doi: 10.3791/55496
March 18th, 2017

Summary March 18th, 2017

G4 Resolvase1 binds to G-quadruplex (G4) structures with the tightest reported affinity for a G4-binding protein and represents the majority of the G4-DNA unwinding activity in HeLa cells. We describe a novel protocol that harnesses the affinity and ATP-dependent unwinding activity of G4-Resolvase1 to specifically purify catalytically active recombinant G4R1.

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