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活性 秀丽隐杆线 虫核提取物的分离及重建用于 体外 转录
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Isolation of Active Caenorhabditis elegans Nuclear Extract and Reconstitution for In Vitro Transcription
DOI:

06:09 min

August 11, 2021

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Chapters

  • 00:05Introduction
  • 00:23Animal and Balch Homogenizer Preparation
  • 01:41Animal Collection
  • 02:21Fractionation
  • 04:57Results: Quality Assessment of Nuclear Extract
  • 05:27Conclusion

Summary

Automatic Translation

在这里,我们描述了从幼虫4 C. elegans 中分离活性核提取物并在 体外 系统中可视化转录活性的详细方案。

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