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Journal
/
Biochemistry
/
活性
秀丽隐杆线
虫核提取物的分离及重建用于
体外
转录
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Isolation of Active
Caenorhabditis elegans
Nuclear Extract and Reconstitution for
In Vitro
Transcription
Please note that all translations are automatically generated.
Click here for the English version.
活性
秀丽隐杆线
虫核提取物的分离及重建用于
体外
转录
DOI:
10.3791/62723-v
•
06:09 min
•
August 11, 2021
•
Phillip Wibisono
,
Jingru Sun
1
Department of Translational Medicine and Physiology, Elson S. Floyd College of Medicine
,
Washington State University
Chapters
00:05
Introduction
00:23
Animal and Balch Homogenizer Preparation
01:41
Animal Collection
02:21
Fractionation
04:57
Results: Quality Assessment of Nuclear Extract
05:27
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们描述了从幼虫4
C. elegans
中分离活性核提取物并在
体外
系统中可视化转录活性的详细方案。
Tags
Caenorhabditis Elegans
Nuclear Extract
In Vitro Transcription
Balch Homogenizer
Hypotonic Buffer
Hypertonic Buffer
Synchronization
L1 Animals
L4 Stage
OP50
Centrifugation
Homogenization
Tungsten Carbide Ball
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