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Biochemistry

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JoVE Journal Biochemistry
用放射性标记的ATP测定蛋白激酶活性
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用放射性标记的ATP测定蛋白激酶活性

Article DOI: 10.3791/55504
May 26th, 2017 •
1, 1, 1
1Department of Pharmacology, University of Texas Southwestern Medical Center

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May 26th, 2017

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蛋白激酶是对于细胞间和细胞内信号转导至关重要的高度进化的信号酶和支架。我们提出了通过使用放射性标记的三磷酸腺苷（[γ- 32 P] ATP）测量激酶活性的方案，这是一种有助于阐明细胞信号调节的可靠方法。

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