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JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology
Inductie van mesenchymale-epitheliale Transities sarcoomcellen
 
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Inductie van mesenchymale-epitheliale Transities sarcoomcellen

Article doi: 10.3791/55520
April 7th, 2017 •
1, 1, 1, 1, 2, 3, 4, 1, 1, 5, 2, 4, 1
1Department of Medicine, Duke University, 2Department of Bioengineering, Rice University, 3Department of Molecular Genetics and Microbiology, Duke University, 4Solid Tumor Program and the Duke Prostate Center, Duke University Medical Center, 5Duke University Medical Center

Summary
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April 7th, 2017

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We presenteren hier een celkweek werkwijze voor het induceren mesenchymale-epitheliale overgangen (MET) sarcoom cellen op basis van gecombineerde ectopische expressie van microRNA-200 familieleden en grainyhead-achtig 2 (GRHL2). Deze methode is geschikt voor een beter begrip van de biologische gevolgen van de fenotypische plasticiteit in de agressiviteit van kanker en behandelingen.

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