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Journal
/
Biochemistry
/
脑膜分离法<em>体检</em评估亚突触蛋白定位的方法
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Brain Membrane Fractionation: An
Ex Vivo
Approach to Assess Subsynaptic Protein Localization
Please note that all translations are automatically generated.
Click here for the English version.
脑膜分离法<em>体检</em评估亚突触蛋白定位的方法
DOI:
10.3791/55661-v
•
09:49 min
•
May 12, 2017
•
Xavier Morató*
1,2
,
Marc López-Cano*
1,2
,
Paula M. Canas
,
Rodrigo A. Cunha
,
Francisco Ciruela
2
1
Unitat de Farmacologia, Departament Patologia i Terapèutica Experimental, Facultat de Medicina, IDIBELL
,
Universitat de Barcelona, L’Hospitalet de Llobregat
,
2
Institut de Neurociències
,
Universitat de Barcelona
,
3
Center for Neurosciences of Coimbra, Institute of Biochemistry, Faculty of Medicine
,
University of Coimbra
Chapters
00:00
Title
00:18
Introduction
02:22
Obtaining Mouse Brain Synaptosomes Using a Discontinuous Sucrose Gradient
04:37
Pre-post and Extra-synaptic Fraction Isolation
06:52
Analyze Samples and Validate Fractionation
07:26
Representative Results
08:53
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们提出一个脑膜分离方案,代表一个强大的程序来分离属于不同突触室的蛋白质。
Tags
Brain Membrane Fractionation
Synaptic Compartments
Synaptic Transmission
Electron Microscopy
Hippocampus
Homogenization
Sucrose Calcium Solution
Centrifugation
Synaptosome
Subsynaptic Compartments
Detergent Solubilization
PH
Immunoblot Analysis
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