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Journal / Biochemistry / Purificación de proteínas de superficie celular…
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Biochemistry
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JoVE Journal Biochemistry
Purificación de proteínas de superficie celular biotiniladas de
Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy
Journal (Biochemistry)
Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy
Crystallization of Heterodimeric ABCG5/G8 Protein in Lipidic Bicelle Using Hanging Drop Vapor Diffusion (Video) | JoVE
Journal (Biochemistry)
Crystallization of Heterodimeric ABCG5/G8 Protein in Lipidic Bicelle Using Hanging Drop Vapor Diffusion (Video) | JoVE
 
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Purificación de proteínas de superficie celular biotiniladas de

Article DOI: 10.3791/55747-v • 12:03 min • July 23rd, 2017
July 23rd, 2017 •
1, 1,2, 1
1Queensland Alliance for Agriculture & Food Innovation, The University of Queensland, 2Centre for Comparative Genomics, Murdoch University

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Se utilizó una metodología basada en gradiente de densidad de densidad modificada para aislar células epiteliales del tejido intestinal de Rhipicephalus microplus . Las proteínas unidas a la superficie se biotinilaron y se purificaron a través de perlas magnéticas de estreptavidina para su utilización en aplicaciones aguas abajo.

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Bioquímica Número 125 Proteínas de superficie celular biotinilación garrapatas microplus células epiteliales intestinales
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